MyGen™-UltimateSens™ NGS
Sequencing Service Products:
FFPE Sample NGS
NGS using FFPE slide is proven to be a very valuable resource for retrospective studies across long period of time and providing opportunity to correlate disease state, outcome and tissue morphology to genotype (DNA) and/or expression (RNA). However, it is challenging to get intact information from FFPE samples because of the severe degradation, damage and biological modification occured within such samples during years of storage in the air at variable ambient temperatures. With Omics Technologies’s unique MyGen™-UltimateSens™ FFPE NGS service, NGS sequencing can be accomplished with as few as one FFPE slide.
| FFPE NGS Key Metrics | |
|---|---|
| Regions Analyzed | Whole Genome/ Whole Exome/ Any Targeted Regions |
| Sequencing Method | Illumina next generation sequencing |
| Bioinformatics | Proprietary methods and visual inspection |
| Assay Sensitivity | >95% |
| Assay Specificity | >99.99% |
| Sequencing Coverage | 150x |
| Turn-around Time | 6-8 weeks |
| Sample Types | FFPE slides/ DNA Extract from FFPE slides |
| DNA Input Required | 1 ng (minimum 0.05 ng) |
Damaged DNA NGS
The quest to trace the roots of the human race has long motivated scientists into the sequences of ancient DNA extracted from skeletal remains unearthed by archaeologists. However, ancient DNA typically bears signs of fraying and fragmentation inflicted by exposure to the elements over great gulfs of time, posing challenges to a successful NGS sequencing. Omics Technologies's MyGen™-UltimateSens™ was initially invented to concstuct NGS libraries with human remains 30,000 years old. Our genomics team is specialized in handling toughest ancient and damaged DNA samples and provide you best result in the field of NGS sequencing.
| Damaged DNA NGS Key Metrics | |
|---|---|
| Regions Analyzed | Whole Genome/ Whole Exome/ Any Targeted Regions |
| Sequencing Method | Illumina next generation sequencing |
| Bioinformatics | Proprietary methods and visual inspection |
| Assay Sensitivity | >95% |
| Assay Specificity | >99.99% |
| Sequencing Coverage | 150x |
| Turn-around Time | 6-8 weeks |
| Sample Types | fossils, human/animal remains, FFPE slides, and body fluid remains |
| DNA Input Required | 1 ng (minimum 0.05 ng) |
Ultra-Low Input NGS
In a NGS seqeuncing project, it is very common to fall into the situation of being lack of enough materials for sequencing. Samples that are collected for deep study, such as NGS sequencing, are usually precious with very limited amount available. Sometimes, you have enough materials to start with, however, after several rounds of library preparations, you still can not get NGS datasets with desired quality, and now only very few samples are left for further test. It is the time to contact Omics Technologies and learn more about our MyGen™-UltimateSens™ NGS technology, a NGS library construction method WITHOUT EXCESSIVE PCR AMPLIFICATION.
| Ultra-Low Input NGS Key Metrics | |
|---|---|
| Regions Analyzed | Whole Genome/ Whole Exome/ Any Targeted Regions |
| Sequencing Method | Illumina next generation sequencing |
| Bioinformatics | Proprietary methods and visual inspection |
| Assay Sensitivity | >95% |
| Assay Specificity | >99.99% |
| Sequencing Coverage | 150x |
| Turn-around Time | 6-8 weeks |
| Sample Types | Frozen tissue, FFPE slides, cell lines, body fluids, and xenograft |
| DNA Input Required | 1 ng (minimum 0.05 ng) |
Targeted NGS
We offer high-quality and rapid target capture service to our customers for their targeted sequencing or re-sequencing projects. Using Agilent SureSelect Target Enrichment Method combined with a modified version of UltimateSens™ , we perform highly sensitive genomic target capture from as low as 0.5 ng DNA library. Based on our combined > 40 years of expertise in genomics fields, we also provide customized target capture probe design service for your research purpose. Simply list the genomic regions or genes you are interested in, we will send you the chip/probe design with our proved ultra high capturing efficacy.
| Targeted NGS Key Metrics | |
|---|---|
| Regions Analyzed | Whole Exome/ Any Panel of Genes/ Any Targeted Regions |
| Sequencing Method | Illumina next generation sequencing |
| Bioinformatics | Proprietary methods and visual inspection |
| Assay Sensitivity | >95% |
| Assay Specificity | >99.99% |
| Sequencing Coverage | 150x |
| Turn-around Time | 6-8 weeks |
| Sample Types | Frozen tissue, FFPE slides, cell lines, body fluids, and xenograft |
| DNA Input Required | 5 ng (minimum 0.5 ng) |
I have extensive experience on proteomic research, therefore my requirements are very high. I have to tell that I am fully satisfied with the provided service and results. I was also very positively impressed that the price was reasonable for the service.
Ira-Ida Skvortsova, MD.,
Professor, Head of the Laboratory for Translational Research in Radiation Oncology, Innsbruck Medical University, Austria
We have had the pleasure of working with Ryan and his team at Omics Technologies on several large projects and they have provided exceptional service.
Laura E. Edsberg, Ph.D.,
Director, Center for Wound Healing Research
Natural & Health Sciences Research Center, Daemen College
They worked hard to help us get the best results for our projects. They are available to answer questions at any time and provide excellent guidance and advice during the projects. I plan to work with them on our future projects.
Laura E. Edsberg, Ph.D.,
Director, Center for Wound Healing Research
Natural & Health Sciences Research Center, Daemen College
-
Phone:
+1 888-206-0066 (TOLL FREE) -
Email:
info@omicstech.com
