PTM Proteomics

PTM Proteomics Identification and Quantification Services

The services here are designed to study all identifiable post-translational modifications. We will designed a customized method to enrich your specific PTMs and will do the following Mass Spectrometry based identifications and quantifications for you. This is a totally customized service, designed specifically for you based on your research goal and your sample.

1) Sample preparation – Cell or tissue lysis, biofluid (plasma or serum) depletion; protein quantification on all samples.

2) Sample digestion – Reduction and alkylation followed by in-solution trypsin digestion and C18 cleaning before labeling.

3) PTM-peptide enrichment – Enrichment of modified peptides according to your choice. We will apply our patented Mass Spectrometry friendly technique, My-IPElu, to fully recover your peptides.

4) (Optional) Sample labeling – Reagents are available that allow the labeling of 2 through 10 samples individuallysimultaneously.

5) (Optional) Sample pooling – Equal amounts (according to total protein quantification) of labeled samples are pooled together to allow unbiased downstream purification and Mass Spectrometry analysis.

6) SCX cleaning – HPLC cleaning and fractionation of pooled sample through off-line SCX.

7) 2D fractionation – bRPLC separation of the SCX cleaned samples into 96 fractions, and further pooled into 24 fractions according to MyProt™-iPooling algorithm (based on peptide hydrophobicity, IEP, etc.)

8) Nano LC/MS/MS – 50 nL/min liquid chromatography to enable super high ionization efficiency for downstream Mass Spectrometry Analysis using the state-of-the-art Orbitrap Mass Spectrometer.

9) Protein profiling – Protein database searching with Mascot, Sequest HT, PEAKS de novo approaches, etc.

10) Data analysis – Data validation, visualization and quantification using Omics Technologies' unique R package and scripts.

11) Report generation – Report will be sent to you in Excel format as well as a summary in pdf format. We will also provide you any detail you need for your papers' MATERIALS AND METHOD section. We will make sure you understand your result.

1, 2D gel spots, SDS-PAGE bands

2, Cell Lysates and Tissue Lysates

3, Biofluids, such as plasma*, serum*, saliva, tear, etc.

*We provide High Abundance Protein Depletion Service to significantly (100-500 folds) increase the depth of your biofluid proteomics analysis by removing top abundant proteins from your samples. Read more for details​.

4, FFPE slide/ FFPE extract

5, Customized sample types (please contact us to discuss)

Please refer to our Omics Technologies Proteomics Sample Preparation and Shipping Guidelines for instructions on how to prepare your samples with less contamination for Mass Spectrometry Analysis.