Protein Identification / Mapping
Protein Identification:
Your protein samples (a protein solution or a piece of your SDS-PAGE gel) are digested with Trypsin and peptides are analyzed by world's most advanced Orbitrap LC-MS/MS. Fingerprints of your proteins are generated and matched to protein sequence database. Report will be sent to you in Excel format as well as a summary in PDF format. We will also provide you any details you need for your papers' MATERIALS AND METHODS section. We will make sure you understand your result and help you with your paper writing with free following-up services.
Protein Mapping:
Your protein samples (a protein solution or a piece of your SDS-PAGE gel) are digested with 3 enzymes (by default: Trypsin, Chymotrypsin and Elastase) individually, or any enzymes you choose from the table below. Peptides are analyzed by world's most advanced Orbitrap LC-MS/MS. Fingerprints of your proteins generated by digestion with each enzyme are matched to protein sequence database individually. Report will be sent to you in Excel format as well as a summary of individual enzyme digestion coverage and total coverage in PDF format. We will also provide you any details you need for your papers' MATERIALS AND METHODS section. We will make sure you understand your result and help you with your paper writing with free following-up services.
List of Proteases You Can Choose From:
(table adopted from Promega, our exclusive protease provider)
Sample types we accept:
1, 2D gel spots, SDS-PAGE bands
2, Cell Lysates and Tissue Lysates
3, Biofluids, such as plasma*, serum*, saliva, tear, etc.
*We provide High Abundance Protein Depletion Service to significantly (100-500 folds) increase the depth of your biofluid proteomics analysis by removing top abundant proteins from your samples. Read more for details.
4, FFPE slide/ FFPE extract
5, Customized sample types (please contact us to discuss)
Please refer to our Omics Technologies Proteomics Sample Preparation and Shipping Guidelines for instructions on how to prepare your samples with less contamination for Mass Spectrometry Analysis.
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Phone:
+1 888-206-0066 (TOLL FREE) -
Email:
info@omicstech.com